Affinity chromatography of hepatitis B antigen.
نویسندگان
چکیده
منابع مشابه
EXPRESSION OF HEPATITIS B SURFACE ANTIGEN IN SACCHAROMYCES CEREVISIAE
The genome of HB V virus of serotype ayw cloned in pBR322 and expression shuttle vector p YES2 were used for construction of the HBsAg chimeric genes and their expression in Saccharomyces cerevisiae. Two recombinant plasmids were constructed. One of them contained the coding sequences for the major polypeptide of surface antigen. Another construct carried the major polypeptide with the pre-S2 a...
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Background: Plasminogen is one of the compounds derived from human plasma. Activation of plasminogen produces plasmin. Plasmin is able to lyse fibrinogen, fibrin, and some other human plasma proteins. The aim of the present work was to study the separation of human plasminogen by affinity chromatography using gel lysine Sepharose. Materials and Methods: Normal human plasma was used as the...
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Affinity chromatography was performed to obtain highly purified antigen from equine infectious anemia (EIA) virus. After crude antigen was concentrated by polyethylene glycol precipitation of culture fluids from equine dermal cells persistently infected with EIA virus, and after the virus was disrupted with ether, it was added to a column of cyanogen bromide-activated Sepharose 4B to which EIA-...
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exposure (Prince, Hargrove, Szmuness, Cherubin, Fontana, and Jeffries, 1970; Papaevangelou, Kourea, and Tsoukas, 1971; Fulford, Dane, Catterall, Woof, and Denning, 1973). A high frequency of HB Ag-positive hepatitis cases was also found among homosexuals (Vahrman, 1973) and evidence has been presented that hepatitis B may spread between sexual partners (Hersh, Melnick, Goyal, and Hollinger, 197...
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We have identified an acceptor site on HIV gp120, where foreign protein sequences can be inserted while retaining the native conformation of gp120. The resulting hybrids showed dual antigenicity, normal glycosylation, and high affinity binding of the CD4 receptor. This site allows insertion of highly immunogenic proteins such as core antigen of hepatitis B virus. By combining the immunogenicity...
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ژورنال
عنوان ژورنال: The Tohoku Journal of Experimental Medicine
سال: 1974
ISSN: 0040-8727,1349-3329
DOI: 10.1620/tjem.113.393